000			02352nam a2200241 4500
001			393928
090			_a393928
100			_a20160523d2014 y0pory50 ba
101			_aeng
200			_aDevelopment and validation of standard operating procedures for measuring microbial populations for estimating a postmortem interval _bRecurso eletrónico _fJeffrey K. Tomberlin ...[et al.]
210			_a[Rockville, MD] _cNational Criminal Justice Reference Service (NCJRS) _d2014
215			_a1 CD-ROM _d12 cm
300			_aResearch report submitted to the U.S. Department of Justice. NIJ Award Number: 2010-DN-BX-K243. Ficheiro de 13,4 MB em formato PDF (82 p.).
330			_aPredicting the postmortem interval of a decedent is a major task of law enforcement. Mostmethods implemented by death investigators rely on qualitative information (i.e., rigor mortis,livor mortis). Microbes represent 99% of somatic cells in and on a human body. No data areavailable on the use of these organisms to predict the time since death of a decedent, though it isknown that certain chemicals, many of which are likely a result of microbial communities, arereleased by decomposing remains in a reliable pattern. Moreover, the effects of microbes oninsect colonization of remains, sometimes the best predictor of a postmortem interval, are notunderstood. Because of a lack of understanding of microbial succession on decomposing humanremains, no standard operating procedures (SOP) for sampling and using this information havebeen developed and validated. We developed a SOP for sampling and pyrosequencing bacteriacommunities on human remains to predict the actual time since death. We conducted a series oflaboratory and field studies to achieve this end. The field studies will implement the SOP forsampling bacteria on decomposing human remains at the Forensic Anthropology ResearchFacility at Texas State University.
606			_aIDENTIFICAÇÃO BIOLÓGICA _9328871
606			_aANÁLISE LABORATORIAL _9326721
606			_aANÁLISE DE VESTÍGIOS _9320649
606			_aIDENTIFICAÇÃO DE CADÁVER _9326379
710			_aDEVELOPMENT AND VALIDATION OF STANDARD OPERATING PROCEDURES FOR MEASURING MICROBIAL POPULATIONS FOR ESTIMATING A POSTMORTEM INTERVAL _9358111
955			_xOferta