000			04250nam a2200265 4500
001			393760
090			_a393760
100			_a20160229d2013 y0pory50 ba
101			_aeng
200			_aDesigner amphetamines in forensic toxicology casework _bRecurso eletrónico _fSarah Kerrigan
210			_a[Rockville, MD] _cNational Criminal Justice Reference Service (NCJRS) _d2013
215			_a1 CD-ROM _d12 cm
300			_aResearch report submitted to the U.S. Department of Justice (Award nr. 2008-DN-BX-K126). Ficheiro de 4,00 MB em formato PDF (90 p.). Resumo inserto na publicação.
330			_a"The 2C, 2C-T and DO-series of designer drugs pose a number of challenges to forensic toxicology laboratories. Although these drugs are seized by law enforcement agencies throughout the United States, they are not readily detected in forensic toxicology laboratories. A systematic evaluation of the cross-reactivity of nine commercial enzyme-linked immunosorbent assays (ELISAs) was conducted using eleven psychedelic amphetamines. Cross-reactivity was measured towards 4-bromo-2,5-dimethoxyphenethylamine (2C-B), 2,5- dimethoxyphenethylamine (2C-H), 2,5-dimethoxy-4-iodophenethylamine (2C-I), 2,5-dimethoxy- 4-ethylthiophenethylamine (2C-T-2), 2,5-dimethoxy-4 isopropylthiophenethylamine (2C-T-4), 2,5-dimethoxy-4-propylthiophenethylamine (2C-T-7), 2,5-dimethoxy-4-bromoamphetamine (DOB), 2,5-dimethoxy-4-ethylamphetamine (DOET), 2,5-dimethoxy-4 iodoamphetamine (DOI), 2,5-dimethoxy-4-methylamphetamine (DOM) and 4-methylthioamphetamine (4-MTA). Some cross-reactivity towards 4-MTA was noted, but cross-reactivity towards the ten remaining 2C, 2C-T and DO-series drugs was extremely low (<0.4%), greatly reducing the likelihood of detection during routine screening. As a consequence, laboratories that rely upon immunoassay rather than more broad spectrum chromatographic screening techniques, may fail to detect these powerful psychedelic substances. A procedure for the simultaneous detection of 2C-B, 2C-H, 2C-I, 2C-T-2, 2C-T-7, DOB, DOET, DOI, DOM and 4-MTA in urine was developed and optimized using gas chromatography/mass spectrometry (GC/MS) and solid phase extraction (SPE). Limits of detection for all analytes were 2 – 10 ng/mL and limits of quantitation were 10 ng/mL or less. Additional reference materials were made available, allowing liquid chromatography-tandem mass spectrometry (LC/MS/MS) to be used for the simultaneous detection of fifteen psychedelic amphetamines in blood and urine. The following additional designer drugs were included in the assay: (2C-T-4, 2,5-dimethoxy-4-chlorophenethylamine (2C-C), 2,5-dimethoxy-4-methylphenethylamine (2C-D), 2,5-dimethoxy-4-ethylphenethylamine (2C-E), and 2,5-dimethoxy-4-chloroamphetamine (DOC). Assay performance was evaluated in terms of analytical recovery, limit of detection, limit of quantitation, precision, accuracy, linearity, matrix effect and interferences. The techniques described allow for the simultaneous detection of fifteen psychostimulants at sub-ng/mL concentrations. Despite their use among recreational drug users, the prevalence of these drugs in forensic toxicology investigations is not widely reported or understood due to variety of analytical and pharmacological challenges. In a study of 2,021 adjudicated forensic casework specimens, 2,5-dimethoxy-4-iodoamphetamine (DOI) was detected in two cases at a concentrations of 1 and 2 ng/mL in urine. The drug is often sold in the form of LSD-like “blotters”. DOI is a low-dose (1.5-3 mg) psychostimulant that is renowned for its powerful psychedelic effects. This is the first report of DOI to date. The low concentrations and highly sensitive and techniques that are required highlights the significant analytical challenges faced by forensic laboratories when detecting many of the psychedelic amphetamines."
606			_aANFETAMINA _9343626
606			_aTOXICOLOGIA FORENSE _9100154
606			_aANÁLISE LABORATORIAL _9326721
606			_aDETECÇÃO DE DROGA _9320535
606			_aQUÍMICA FORENSE _9343048
700			_aKERRIGAN _bSarah _926441
955			_xOferta