000			03417nam a2200265 4500
001			393614
090			_a393614
100			_a20151117d2013 y0pory50 ba
101			_aeng
200			_aDevelopment and testing of a rapid multiplex assay for the identification of biological stains _bRecurso eletrónico _fKevin M. Legg
210			_a[Rockville, MD] _cNational Criminal Justice Reference Service (NCJRS) _d2013
215			_a1 CD-ROM _d12 cm
300			_aResearch report submitted to the U.S. Department of Justice. NIJ Award Number: 2011-CD-BX-0205. A dissertation presented to the Faculty of Natural Sciences and Mathematics of the University of Denver in partial fulfillment of the requirements for the Degree Doctor of Philosophy. Ficheiro de 8,66 MB em formato PDF (250 p.).
330			_aWhile DNA profiling makes it possible to individualize biological stains, the identification of the stain itself can present forensic serologists with a significant challenge. Current antibody- and enzyme activity-based assays used by forensic practitioners for biological stain identification yield only presumptive results. Positive results with non-target body fluids or cross-reactivity with non-human sources has also been well documented. Some tests can consume unacceptable quantities of precious evidence while for some body fluids (vaginal fluid and menstrual blood) there are simply no available tests at all. This research presented here aims to develop and rigorously test a fast, accurate, and sensitive multiplex assay for the simultaneous identification of saliva, semen, urine, peripheral blood, menstrual blood, and vaginal secretions. This research is based on the following three research phases: 1. Biomarker Identification – Utilizing multidimensional protein separation technologies, bioinformatics tool, and tandem mass spectrometry a database of fluid-specific candidate markers will be developed for each body fluid. 2. Biomarker Verification – Using the most promising candidates from Phase 1 a targeted multiplex assay will be developed on a Quadruple Time-of-Flight mass spectrometer to verify the specificity of these candidate biomarkers with single source laboratory samples as well as single and mixed source casework type samples. 3. Prototype Validation – Development and testing of a case working laboratory prototype assay on a triple quadrupole mass spectrometer using single and mixed source casework-type samples. This work has the potential to significantly improve the accuracy and sensitivity of forensic serological testing. It will provide practitioners with greatly improved tests for saliva and seminal fluid while also enabling the identification of vaginal secretions for which there are currently no accurate tests. The multiplex design will eliminate the need to perform separate tests on an unknown stain. In short, the successful completion and implementation of this research will provide the forensic community with a powerful tool to aid in criminal investigations.
606			_aANÁLISE DE VESTÍGIOS _9320649
606			_aFLUIDO CORPORAL _9343188
606			_aIDENTIFICAÇÃO BIOLÓGICA _9328871
606			_aANÁLISE LABORATORIAL _9326721
606			_aTESE _9334284
606			_aESTADOS UNIDOS _9334103
700			_aLEGG _bKevin M. _9357915
955			_xOferta