02352nam a2200241 4500 393928 393928 20160523d2014 y0pory50 ba eng Development and validation of standard operating procedures for measuring microbial populations for estimating a postmortem interval Recurso eletrónico Jeffrey K. Tomberlin ...[et al.] [Rockville, MD] National Criminal Justice Reference Service (NCJRS) 2014 1 CD-ROM 12 cm Research report submitted to the U.S. Department of Justice. NIJ Award Number: 2010-DN-BX-K243. Ficheiro de 13,4 MB em formato PDF (82 p.). Predicting the postmortem interval of a decedent is a major task of law enforcement. Mostmethods implemented by death investigators rely on qualitative information (i.e., rigor mortis,livor mortis). Microbes represent 99% of somatic cells in and on a human body. No data areavailable on the use of these organisms to predict the time since death of a decedent, though it isknown that certain chemicals, many of which are likely a result of microbial communities, arereleased by decomposing remains in a reliable pattern. Moreover, the effects of microbes oninsect colonization of remains, sometimes the best predictor of a postmortem interval, are notunderstood. Because of a lack of understanding of microbial succession on decomposing humanremains, no standard operating procedures (SOP) for sampling and using this information havebeen developed and validated. We developed a SOP for sampling and pyrosequencing bacteriacommunities on human remains to predict the actual time since death. We conducted a series oflaboratory and field studies to achieve this end. The field studies will implement the SOP forsampling bacteria on decomposing human remains at the Forensic Anthropology ResearchFacility at Texas State University. IDENTIFICAÇÃO BIOLÓGICA 328871 ANÁLISE LABORATORIAL 326721 ANÁLISE DE VESTÍGIOS 320649 IDENTIFICAÇÃO DE CADÁVER 326379 DEVELOPMENT AND VALIDATION OF STANDARD OPERATING PROCEDURES FOR MEASURING MICROBIAL POPULATIONS FOR ESTIMATING A POSTMORTEM INTERVAL 358111 Oferta 0 PJCD_258_000000000000000 2022-06-06 PJCD258 426466 PJ PJ PJ1CD258_41 PJCD258 2022-06-06 0 MO Oferta