02170nam a2200181   4500001000700000100004100007101000800048200019300056210007900249215002000328300014400348330123400492606003401726606002701760606002901787606003501816710013701851393928  a20160523d2014       y0pory50      ba  aeng  aDevelopment and validation of standard operating procedures for measuring microbial populations for estimating a postmortem intervalbRecurso eletrónicofJeffrey K. Tomberlin ...[et al.]  a[Rockville, MD]cNational Criminal Justice Reference Service (NCJRS)d2014  a1 CD-ROMd12 cm  aResearch report submitted to the U.S. Department of Justice. NIJ Award Number: 2010-DN-BX-K243. Ficheiro de 13,4 MB em formato PDF (82 p.).  aPredicting the postmortem interval of a decedent is a major task of law enforcement. Mostmethods implemented by death investigators rely on qualitative information (i.e., rigor mortis,livor mortis). Microbes represent 99% of somatic cells in and on a human body. No data areavailable on the use of these organisms to predict the time since death of a decedent, though it isknown that certain chemicals, many of which are likely a result of microbial communities, arereleased by decomposing remains in a reliable pattern. Moreover, the effects of microbes oninsect colonization of remains, sometimes the best predictor of a postmortem interval, are notunderstood. Because of a lack of understanding of microbial succession on decomposing humanremains, no standard operating procedures (SOP) for sampling and using this information havebeen developed and validated. We developed a SOP for sampling and pyrosequencing bacteriacommunities on human remains to predict the actual time since death. We conducted a series oflaboratory and field studies to achieve this end. The field studies will implement the SOP forsampling bacteria on decomposing human remains at the Forensic Anthropology ResearchFacility at Texas State University.  aIDENTIFICAÇÃO BIOLÓGICA  aANÁLISE LABORATORIAL  aANÁLISE DE VESTÍGIOS  aIDENTIFICAÇÃO DE CADÁVER  aDEVELOPMENT AND VALIDATION OF STANDARD OPERATING PROCEDURES FOR MEASURING MICROBIAL POPULATIONS FOR ESTIMATING A POSTMORTEM INTERVAL