01830nam a2200205   4500001000700000100004100007101000800048200015900056210002500215215002000240300012900260330110300389606000801492606002301500606002101523606002901544700001701573701001601590701001801606393717  a20160127d2015       y0pory50      ba  aeng  aTrace DNA from fingernailsbRecurso eletrónicoeincreasing the success rate of widely collected forensic evidencefDavid Foran, Lisa Hebda, Ashley Doran  a[S.l.]c[s.n.]d2015  a1 CD-ROMd12 cm  a"This document is a research report submitted to the U.S. Department of Justice".Ficheiro de 1,98 MB em formato PDF (64 p.).  aIntroduction. Methods. Molecular Techniques. DNA Extraction and Purification. Exogenous DNA Quantification via Real-Time PCR. Autosomal STR and Y-STR Analyses. Initial Scratching Experiments. Comparison of Collection Techniques for Obtaining Exogenous DNA from Nails. Nail Preparation . DNA Collection Techniques. Comparison of Organic and Commercial Kit Extractions. Scratching Using Improved Cell Collection and DNA Extraction Protocols. Ancillary Tests. Transportation of Nail Evidence. Cumulative Swabbing of Fingernails. Effect of Nail Polish on DNA Amplification and Analysis. Source of Endogenous DNA from Fingernails. Statistical Tests. Results. Initial Scratching Experiments. Comparison of Collection Techniques for Obtaining Exogenous DNA from Nails. Comparison of Organic and Commercial Kit Extractions. Scratchings Using Improved Cell Collection and DNA Extraction Protocols. Ancillary Tests. Transportation of Nail Evidence. Cumulative Swabbing of Fingernails. Effect of Nail Polish on DNA Amplification and Analysis. Source of Endogenous DNA from Fingernails. Discussion. References.  aADN  aANÁLISE DE UNHAS  aRECOLHA DA PROVA  aANÁLISE DE VESTÍGIOS  aFORANbDavid  aHEBDAbLisa  aDORANbAshley