02463nam a2200205   4500001000700000100004100007101000800048200010400056210005200160215002000212300004800232330184000280606001702120606000802137606002502145606002702170606001902197700002002216701002102236393223  a20150428d2015       y0pory50      ba  aeng  aPost-coital DNA recovery studybRecurso eletrónicoefinal reportfPatricia Speck, Jack Ballantyne  aWashingtoncNational Institute of Justiced2015  a1 CD-ROMd12 cm  aFicheiro de 2,23 MB em formato PDF (119 p.)  aAbstract. Executive Summary. Table of Contents. Introduction. Statement of the Problem. DNA Recovery. Semen and Sperm. Male Contribution to Cervical-Vaginal Sperm Retention. Vulva and Vagina. Cervix. Persistence of DNA on Other Sites. Forensic Laboratory. Summary. Research Aims, Purpose and Objectives. Methods. Research Design. Methods, Materials and Procedures. Focus Group. Bayesian Review . DNA Recovery Protocol. Recruitment and Consent. The Protocol Process. Protection of Human Subjects and Confidentiality. Recruitment Consenting Couples. Participant recruitment. Inclusion criteria. Exclusion criteria. Ethical Considerations. Confidentiality Bias. Kit Distribution Process. Quality assurance, packaging, and storage. USPO mailing requirement. Collection Protocol for Post-coital Interval Samples. Collectors. Speculum insertion and contamination. Sampling. Labeling. Documentation. Daily activities, conditions, condom and coitus history. Documentation of clinical appearance. Laboratory Method. DNA isolation. Purification. Quantitation. Y chromosome-specific nested PCR pre-amplification. Y-STR amplifications. PCR product detection - capillary electrophoresis. Data Processing. Summary of Post-Coital DNA Recovery Collection Protocol. Statement of Results. Qualitative Analysis. Focus group. Focus group suggested changes in protocol. Bayesian review results. Descriptive Results. Post-coital DNA kits. Demographics. Inferential Results. DNA recovery. Hormonal Birth Control and Menstruation. Standard Y-STR. Enhanced Y-STR. Assumptions and Limitations. Assumptions. Limitations. Tables. Figures. Conclusions. Discussion of Findings. Implications for Policy and Practice. Implications for Further Research. Recommendations. References. Dissemination of Research Findings. Publications. Presentations. Definition of Terms.  aCRIME SEXUAL  aADN  aEXAME MÉDICO-LEGAL  aANÁLISE LABORATORIAL  aESTADOS UNIDOS  aSPECKbPatricia  aBALLANTYNEbJack