01449nam a2200193 4500001000700000100004100007101000800048200015200056210007800208215002000286300012800306330066500434606002901099606000801128606002701136606002201163700002401185710004601209392949 a20150112d2014 y0pory50 ba aeng aA rapid, efficient, and effective assay to determine species origin in biological materialsbRecurso eletrónicoefinal reportfJared M. Latiolais a[Lorton, VA]cNational Criminal Justice Reference Service (NCJRS)d[2014] a1 CD-ROMd12 cm aResearch report submitted to the U.S. Department of Justice by The Bode Technology Group, Inc., NIJ Grant #2007-DN-BX-K144. aExecutive summary. Introduction. Literature review. Project strategy. Assay evaluation, interpretation, control DNA. Overview of specific goals. Summary of progress on goals. Executive summary conclusion and future directions. Experimental details and main findings. Sample gathering and data matrix generation. Bioinformatic analysis and canonical SNP identification. SBE and PCR primer development. SBE primer specificity and cross reactivity testing. PCR primer multiplexing. SBE primer multiplexing. Two species mixtures. Three species mixtures. Nine species mixtures. Reaction troubleshooting. Conclusions. Key recommendations. Bibliography. Appendix A. aANÁLISE DE VESTÍGIOS aADN aANÁLISE LABORATORIAL aCIÊNCIA FORENSE aLATIOLAISbJared M. aESTADOS UNIDOSbThe Bode Technology Group