01243nam a2200169   4500001000700000100004100007101000800048200008200056210003700138215002000175300028800195330051000483606002700993606000801020606002201028700002301050391203  a20121012d2012       y0pory50      ba  aeng  aTaq mutants engineered for forensicsbRecurso eletrónicofMilko Kermekchiev  aSaint Louis, MOced. do a.d2012  a1 CD-ROMd12 cm  aResearch report submitted to the U.S. Department of Justice, NIJ Award No. 2008-DN-BX-K299. Resumo inserto na publicação. Ficheiro de 1,58 MB em formato PDF (43 p.). Disponível também em: https://www.ncjrs.gov/pdffiles1/nij/grants/239074.pdf. Acedido a 12 de outubro de 2012.  aMajor problems with PCR-based forensic tests are false negative results and low sensitivity caused by residual blood, soil or other PCR inhibitors present in the sample. We proposed to apply our novel genetically-engineered mutants of Taq DNA polymerase, highly resistant to PCR inhibitors, to direct DNA analysis of forensic samples. In many cases, this approach can eliminate the need to purify DNA prior to PCR and decrease the time, lower the cost, and increase the efficiency of forensic DNA testing.  aANÁLISE LABORATORIAL  aADN  aCIÊNCIA FORENSE  aKERMEKCHIEVbMilko