02010nam a2200193 4500001000700000100004100007101000800048200020500056210002800261215002000289300024800309330112300557606002901680606000801709606002901717606002101746700002601767701002301793390983 a20120601d2012 y0pory50 ba aeng aInvestigations on the use of Samplematrix to stabilize crime scene biological samples for optimized analysis and room temperature storagebRecurso eletrónicofKatherine A. Roberts, Donald J. Johnson a[S.l.]ced. do a.d2012 a1 CD-ROMd12 cm aResearch report submitted to the U.S. Department of Justice Award no. 2007-DN-BX-K172. Ficheiro de 7,86 MB em formato PDF (296 p.). Disponível também em: https://www.ncjrs.gov/pdffiles1/nij/grants/237838.pdf. Acedido a 01 de junho de 2012. aStorage and preservation of biological evidence are key forensic issues that are currentlyunder investigation in anticipation of more cost saving and effective stabilization methods. Onceevidence has been identified and collected at a crime scene, it is typically stored for analysislater. Storing biological evidence at subzero temperatures is one of the current methodsemployed to prevent DNA degradation until time of analysis; however, previous studies haveshown that freezing biological evidence does not completely cease the degradation process. Inaddition, it has been shown that repeated freezing and thawing of biological evidence may add tothe degradation effects. The research presented here evaluates a proprietary platform technologyfor the dry storage of biological materials at ambient temperature. The technology behind thisroom temperature stability is primarily adopted from natural principles and is based on the DNAstabilizing properties of small molecules such as trehalose. Three non-lysis formulations (SM1,SM2, and SM3) and one lysis formulation were evaluated with approximately 7,500 samples. aPRESERVAÇÃO DA PROVA aADN aANÁLISE DE VESTÍGIOS aBIOLOGIA FORENSE aROBERTSbKatherine A. aJOHNSONbDonald J.