01414nam a2200193   4500001000700000010001500007100004100022101000800063200009300071210004400164215002800208225003200236330085600268606002201124606001501146606002401161606000801185606002701193383774  a1881299236  a20060531d2000       y0pory50      ba  aeng  aDNA typing protocolsemolecular biology and forensic analysisfBruce Budowle... [et al.]  aNatick, MAcEaton Publishingdcop. 2000  avii, 304 p.cil.d26 cm  aBioForensic Sciences Series  aChapter 1 - Introduction. Chapter 2 - Sample collection and storage. Chapter 3 - Extraction of DNA. Chapter 4 - Determination of the quantity of DNA. Chapter 5 - Restriction fragment-length polymorphism typing of variable number tandem repeat loci. Chapter 6 - PCR-based analyses: allele-specific oligonucleotide assays. Chapter 7 - Electrophoretic separation and allele detection by silver staining for the Loci DS80 and amelogenin. Chapter 8 - Short tandem repeat loci typing using polyacrylamide gel electrophoresis and decoupled fluorescent scanner detection. Chapter 9 - Capillary electrophoresis for short tandem repeat loci typing and real time fluorescent detection. Chapter 10 - PCR-based analysis: mithocondrial DNA sequencing. Appendix 1 - Quality assurance standards. Appendix 2 - Allele frequency data. Appendix 3 - Reagents and supplies.  aCIÊNCIA FORENSE  aGENÉTICA  aMARCADOR GENÉTICO  aADN  aANÁLISE LABORATORIAL