02062nam a2200217 4500 378129 378129 20210621u y0pory50 ba eng Identification and determination of opioid peptides in biological material using the LC-QTOF-MS/MS technique Recurso eletrónico Magdalena PoPławska ...[et al.] Ficheiro de 520 KB em formato PDF. 1. Introduction. 2. Case report. 3. Materials and methods. 3.1. Reagents and reference materials. 3.2. Analytical material. 3.3. Equipment and analytical parameters. 3.4. Qualitative analyses. 3.5. Quantitative analysis of deltorphin I and II in the secretion of Phyllomedusa bicolor. 3.6. Quantitative analysis of deltorphin I and II in blood. 3.7. Isolation of deltorphins from blood. 3.8. Preparation of samples for analysis. 3.9. Solutions for the validation of a method for the determination of deltorphin I and II. 3.10. Validation of the method for the determination of deltorphin I and II in “kambo”. 3.11. Validation of the method for determination of deltorphins in blood. 3.12. Estimating the degradation of deltorphins in blood. 4. results. 4.1. Identification of deltorphin I and II in the analysed sample of secretion from the frog Phyllomedusa bicolor. 4.2. Results obtained for evaluated validation parameters. 4.3. Determination of deltorphin I and deltorphin II concentrations in frog secretion. 4.4. Estimating the degradation of deltorphins in blood. 4.5. Determination of deltorphin I and deltorphin II concentration in the blood sample. 5. Conclusions. 2018 Vol. 116 Problems of Forensic Sciences 1230-7483 p. 357–374 TOXICOLOGIA ANÁLISE LABORATORIAL ESPECTROMETRIA IDENTIFICATION AND DETERMINATION OF OPIOID PEPTIDES IN BIOLOGICAL MATERIAL USING THE LC-QTOF-MS/MS TECHNIQUE 0 PJCD_309_000000000000000 2022-06-06 405910 PJ PJ PJCD 309 2022-06-06 0 AP