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Abstract. Executive Summary. Table of Contents. Introduction. Statement of the Problem. DNA Recovery. Semen and Sperm. Male Contribution to Cervical-Vaginal Sperm Retention. Vulva and Vagina. Cervix. Persistence of DNA on Other Sites. Forensic Laboratory. Summary. Research Aims, Purpose and Objectives. Methods. Research Design. Methods, Materials and Procedures. Focus Group. Bayesian Review . DNA Recovery Protocol. Recruitment and Consent. The Protocol Process. Protection of Human Subjects and Confidentiality. Recruitment Consenting Couples. Participant recruitment. Inclusion criteria. Exclusion criteria. Ethical Considerations. Confidentiality Bias. Kit Distribution Process. Quality assurance, packaging, and storage. USPO mailing requirement. Collection Protocol for Post-coital Interval Samples. Collectors. Speculum insertion and contamination. Sampling. Labeling. Documentation. Daily activities, conditions, condom and coitus history. Documentation of clinical appearance. Laboratory Method. DNA isolation. Purification. Quantitation. Y chromosome-specific nested PCR pre-amplification. Y-STR amplifications. PCR product detection - capillary electrophoresis. Data Processing. Summary of Post-Coital DNA Recovery Collection Protocol. Statement of Results. Qualitative Analysis. Focus group. Focus group suggested changes in protocol. Bayesian review results. Descriptive Results. Post-coital DNA kits. Demographics. Inferential Results. DNA recovery. Hormonal Birth Control and Menstruation. Standard Y-STR. Enhanced Y-STR. Assumptions and Limitations. Assumptions. Limitations. Tables. Figures. Conclusions. Discussion of Findings. Implications for Policy and Practice. Implications for Further Research. Recommendations. References. Dissemination of Research Findings. Publications. Presentations. Definition of Terms.