Taq mutants engineered for forensics [Recurso eletrónico] / Milko Kermekchiev
Idioma: Inglês.Publicação: Saint Louis, MO : ed. do a., 2012Descrição física: 1 CD-ROM; 12 cmAssunto: 326721 | 243660 | 321508Resumo: Major problems with PCR-based forensic tests are false negative results and low sensitivity caused by residual blood, soil or other PCR inhibitors present in the sample. We proposed to apply our novel genetically-engineered mutants of Taq DNA polymerase, highly resistant to PCR inhibitors, to direct DNA analysis of forensic samples. In many cases, this approach can eliminate the need to purify DNA prior to PCR and decrease the time, lower the cost, and increase the efficiency of forensic DNA testing.| Item type | Current location | Call number | Copy number | Status | Date due | Barcode |
|---|---|---|---|---|---|---|
| Monografias | Biblioteca da Polícia Judiciária | PJCD210 (Browse shelf) | PJCD210 | Available | PJ1CD210_51 |
Research report submitted to the U.S. Department of Justice, NIJ Award No. 2008-DN-BX-K299. Resumo inserto na publicação. Ficheiro de 1,58 MB em formato PDF (43 p.). Disponível também em: https://www.ncjrs.gov/pdffiles1/nij/grants/239074.pdf. Acedido a 12 de outubro de 2012.
Major problems with PCR-based forensic tests are false negative results and low sensitivity caused by residual blood, soil or other PCR inhibitors present in the sample. We proposed to apply our novel genetically-engineered mutants of Taq DNA polymerase, highly resistant to PCR inhibitors, to direct DNA analysis of forensic samples. In many cases, this approach can eliminate the need to purify DNA prior to PCR and decrease the time, lower the cost, and increase the efficiency of forensic DNA testing.
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