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Investigations on the use of Samplematrix to stabilize crime scene biological samples for optimized analysis and room temperature storage [Recurso eletrónico] / Katherine A. Roberts, Donald J. Johnson

Autor: ROBERTS, Katherine A.Co-autor: JOHNSON, Donald J.Idioma: Inglês.Publicação: [S.l.] : ed. do a., 2012Descrição física: 1 CD-ROM; 12 cmAssunto: 302776 | 243660 | 320649 | 1092Resumo: Storage and preservation of biological evidence are key forensic issues that are currentlyunder investigation in anticipation of more cost saving and effective stabilization methods. Onceevidence has been identified and collected at a crime scene, it is typically stored for analysislater. Storing biological evidence at subzero temperatures is one of the current methodsemployed to prevent DNA degradation until time of analysis; however, previous studies haveshown that freezing biological evidence does not completely cease the degradation process. Inaddition, it has been shown that repeated freezing and thawing of biological evidence may add tothe degradation effects. The research presented here evaluates a proprietary platform technologyfor the dry storage of biological materials at ambient temperature. The technology behind thisroom temperature stability is primarily adopted from natural principles and is based on the DNAstabilizing properties of small molecules such as trehalose. Three non-lysis formulations (SM1,SM2, and SM3) and one lysis formulation were evaluated with approximately 7,500 samples.
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Monografias Biblioteca da Polícia Judiciária
PJCD200 (Browse shelf) PJCD200 Available PJ1CD200_27

Research report submitted to the U.S. Department of Justice Award no. 2007-DN-BX-K172. Ficheiro de 7,86 MB em formato PDF (296 p.). Disponível também em: https://www.ncjrs.gov/pdffiles1/nij/grants/237838.pdf. Acedido a 01 de junho de 2012.

Storage and preservation of biological evidence are key forensic issues that are currentlyunder investigation in anticipation of more cost saving and effective stabilization methods. Onceevidence has been identified and collected at a crime scene, it is typically stored for analysislater. Storing biological evidence at subzero temperatures is one of the current methodsemployed to prevent DNA degradation until time of analysis; however, previous studies haveshown that freezing biological evidence does not completely cease the degradation process. Inaddition, it has been shown that repeated freezing and thawing of biological evidence may add tothe degradation effects. The research presented here evaluates a proprietary platform technologyfor the dry storage of biological materials at ambient temperature. The technology behind thisroom temperature stability is primarily adopted from natural principles and is based on the DNAstabilizing properties of small molecules such as trehalose. Three non-lysis formulations (SM1,SM2, and SM3) and one lysis formulation were evaluated with approximately 7,500 samples.

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