DNA typing protocols : molecular biology and forensic analysis / Bruce Budowle... [et al.]
Idioma: Inglês.Publicação: Natick, MA : Eaton Publishing, cop. 2000Descrição física: vii, 304 p. : il.; 26 cmColeção: BioForensic Sciences SeriesISBN: 1881299236Assunto: 321508 | 238061 | 343807 | 243660 | 326721Resumo: Chapter 1 - Introduction. Chapter 2 - Sample collection and storage. Chapter 3 - Extraction of DNA. Chapter 4 - Determination of the quantity of DNA. Chapter 5 - Restriction fragment-length polymorphism typing of variable number tandem repeat loci. Chapter 6 - PCR-based analyses: allele-specific oligonucleotide assays. Chapter 7 - Electrophoretic separation and allele detection by silver staining for the Loci DS80 and amelogenin. Chapter 8 - Short tandem repeat loci typing using polyacrylamide gel electrophoresis and decoupled fluorescent scanner detection. Chapter 9 - Capillary electrophoresis for short tandem repeat loci typing and real time fluorescent detection. Chapter 10 - PCR-based analysis: mithocondrial DNA sequencing. Appendix 1 - Quality assurance standards. Appendix 2 - Allele frequency data. Appendix 3 - Reagents and supplies.| Item type | Current location | Call number | Copy number | Status | Date due | Barcode |
|---|---|---|---|---|---|---|
| Monografias | Biblioteca da Polícia Judiciária | PJ27116 | Available | PJ127116 |
Chapter 1 - Introduction. Chapter 2 - Sample collection and storage. Chapter 3 - Extraction of DNA. Chapter 4 - Determination of the quantity of DNA. Chapter 5 - Restriction fragment-length polymorphism typing of variable number tandem repeat loci. Chapter 6 - PCR-based analyses: allele-specific oligonucleotide assays. Chapter 7 - Electrophoretic separation and allele detection by silver staining for the Loci DS80 and amelogenin. Chapter 8 - Short tandem repeat loci typing using polyacrylamide gel electrophoresis and decoupled fluorescent scanner detection. Chapter 9 - Capillary electrophoresis for short tandem repeat loci typing and real time fluorescent detection. Chapter 10 - PCR-based analysis: mithocondrial DNA sequencing. Appendix 1 - Quality assurance standards. Appendix 2 - Allele frequency data. Appendix 3 - Reagents and supplies.
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